DETERMINATION OF THE CONCENTRATION OF NICOTIN AND ITS METABOLITES IN THE URINE SAMPLE OF PASSIVE SMOKERS

Abstract

Ciljevi ispitivanja: Cilj ovog eksperimentalnog rada je bio je pmomoću GC-MS metode kvantitativno odrediti koncentracije nikotina i kotinina i trans-3- hdroksikotinina u biološkim uzorcima urina dobrovoljnih ispitanika prije i poslje odlaksa u noćni klub u kojem je dozvoljeno pušenje. Materijal i metode: U straživanju je sudjelovalo 22 ispitanika, od toga 7 muškaraca i 15 žena. Biološki uzorci urina nepoznath koncentracija nikotina i metabolita pripremljen za analizu ekstrakcijskom metodom tekuće-tekuće (LLE).Za analizu koristili smo GC-MS. Rezultati: Svi uzorci bili su pozitivni na nikotin i metabolite. Srednja vrjednost nikotina, kotinina i 3-OHK prije izloženosti u noćnom klubu kod nepušača iznosila je 8,197 ng/mL, 28,372 ng/mL, 12,303 ng/mL kod žena i 11,235 ng/mL, 25,763 ng/mL, 3,673 ng/mL kod muškaraca. Srednja vrijednost nakon izloženosti iznosila je: 123,373 ng/mL, 56,544 ng/mL, 41,501 ng/mL kod žena i 21,366 ng/mL, 65,234 ng/mL, 14,941 ng/mL kod muškaraca. Zaključci: Koncentracije nikotina i metabolita kod nepušača prije izloženosti bile su značajn manje neko nakon izloženosti. Razine nikotina i metabolita kod pušača značajn su veće nego li kod nepušaća. S obzirom a dužinu izloženost porast koncentracija kod konobara, nepušača za nikotin, kotinin i 3-OHK veći je neko kod muških ispitanika koji su boravili u klubu kao posjetitelji.Aims of the study: The purpose of this experimental work was to quantitatively determine the concentrations of nicotine and cotinine and trans-3-hydroxycotinine in biological urine samples from voluntary subjects before and after their visit to a nightclub in which smoking was allowed, by using the GC-MS method. Material and methods: Twenty-two subjects participated in the research, including 7 men and 15 women. Biological urine samples of unknown nicotine and metabolite concentrations were prepared for analysis by the liquid-liquid extraction (LLE) method. For analysis, we used the GC-MS method. Results: All samples were positive for nicotine metabolites. The mean value of nicotine, cotinine and 3-OHK before exposure at a nightclub in non-smokers was 8,197 ng / mL, 28,372 ng / mL, 12,303 ng / mL in women and 11,235 ng / mL, 25,763 ng / mL, 3,673 ng / mL in men. Mean post-exposure values were: 123,373 ng / mL, 56,544 ng / mL, 41,501 ng / mL in women and 21,366 ng / mL, 65,234 ng / mL, 14,941 ng / mL in men. Conclusions: Concentrations of nicotine and metabolites in non-smokers before exposure were significantly lower than after exposure. The levels of nicotine and metabolites in smokers are significantly higher than in non-smokers. Considering the length of exposure, the increase in concentrations of nicotine, cotinine, and 3-OHK in non-smoker waiters was higher than in male respondents who stayed in the club as visitors