Wetzel, Scott A.

English

University of Montana

University of MontanaScholarWorks at University of MontanaSyllabi Course Syllabi9-2014BIOB 411.01: Immunology LaboratoryScott A. WetzelUniversity of Montana - Missoula, scott.wetzel@umontana.eduLet us know how access to this document benefits you.Follow this and additional works at: https://scholarworks.umt.edu/syllabiThis Syllabus is brought to you for free and open access by the Course Syllabi at ScholarWorks at University of Montana. It has been accepted forinclusion in Syllabi by an authorized administrator of ScholarWorks at University of Montana. For more information, please contactscholarworks@mso.umt.edu.Recommended CitationWetzel, Scott A., "BIOB 411.01: Immunology Laboratory" (2014). Syllabi. 1578.https://scholarworks.umt.edu/syllabi/1578BIOB 4 1 1 -  Im m u n o l o g y  L a b o r a t o r y  F a l l  2 0 1 4 1BIOB 411 IMMUNOLOGY LABORATORYAutumn Semester, 2014Dr. Scott Wetzel (CHCB 216; Phone 243-2168)Lab schedule: 1:10 - 4:00 pm on Thursdays in HS 404 Teaching Assistant-______________________________Date Lab#Page TopicAugust28 1 l Introduction - grading, animal use, safety, etc.September4 2 5 Cells and organs of the immune system- PART 111 3 11 Cells and organs of the immune system- PART 218 4 17 Generating polyclonal antibodies- PART 125 5 19 Immunoagglutination assaysOctober2 6 23 Immunoprecipitation and Electrophoresis9 7 29 Immunoblots16 8 31 Complement fixation assay23 9 34 Immunofluorescence microscopy -— MIDTERM EXAM30 10 37 Passive hemagglutination & Generating polyclonal antibodies -  PART 2November6 11 42 Flow Cytometry13 12 45 E L IS A ................ LAB PAPER OUTLINE DUE20 13 51 Generating polyclonal antibodies- PART 327 - HOLIDAYDecember5 14 54 Complete analysis of antisera, compile data and Lab Paper??? - FINAL EXAM (3:20 to 5:20)12 LAB PAPER & NOTEBOOKS DUE by 5:00 PMGeneral Notes:GRADING- BIOB 411 points are broken down as follows: 200 pts from 2 lab exams (midterm and final), 100 pts from a formal lab report (see below), 70 pts from the lab notebook (5 pts per lab) and 30 points from quizzes. Grades are calculated by 90%, 80%, 70%, and 60% of the total points; which represent the cutoffs for an A, B, C, and D, respectively. Materials turned in late will be reduced by 10% of the total points per day of tardiness. Students missing more than two labs will be required to drop the course.QUIZZES: These are long, and frequently complex laboratory exercises. You MUST read the material and prepare for each lab AHEAD of time (remember this is a 2 credit course). To insure that you come prepared, we will have unannounced quizzes at the beginning of 6 of the first 12 labs during the semester. These quizzes will be worth 5 points and will cover the lab that day.LAB PAPER- Each team will receive a mouse for generating "monospecific" polyclonal antiserum. The protocol and results obtained from this portion of the laboratory (labs 4, 10, 13 & 14) will be summarized by each student in a formal report.BIOB 4 1 1 -  Im m u n o l o g y  L a b o r a t o r y  F a l l  2 0 1 4 2The final paper will be typed and double-spaced. The paper is worth ~ 30% of your lab grade. The style of the report follows the format of the Journal o f Immunologyhttp://www.iimmunol.org/site/misc/authorinstructions.xhtml instructions for full-length articles). It is STRONGLY recommended that you read through these directions before writing your paper. The requirements include:❖ Title Page- Title, name(s), address(es), key words❖ Abstract- A summary of your data (250 words maximum)❖ Introduction- the background and justification for your research❖ Materials and methods- a reiteration (in your own words) of the labs❖ Results- tabulated and graphic data as well as text description. This is a description of your data and includes any calculations of % difference, etc. that isn’t in your figures. Make sure you reference your figures.❖ Discussion- below❖ References- Not required for the report, but If you use a reference other than the lab book you MUST provide a reference, (otherwise this will be plagiarism)G ra d in g  o f  t h e  PAPER is a s  f o l lo w s :Journal of Immunology format and 5-page limit—  10 pts (only the first 5 pages will be graded) Results—quality and data presentation—30 ptsDiscussion—  40 pts. Explain what you might have expected and if your results meet thoseexpectations. Tell the significance of the results. Propose future experiments, etc.Content—20 pts. Grammar, spelling, etc (this is a 2/3 writing course); reflects the presentation and style.OUTLINE OF LAB PAPER- Provide an outline of your lab paper to the TA by the due date on page 1. Describe your ideas for presenting the data and how you will arrange the results in the context of the format above. You will receive formal feedback. You are also required to submit your final paper 1 week before the deadline to receive feedback so that you can revise your paper accordingly before you turn it in to be graded.LAB NOTEBOOK- Each student will maintain a personal and comprehensive lab notebook. Loose-leaf paper is NOT acceptable. Record your data and observations IN PEN for future reference. Be sure and take your notes DURING the lab. Evidence of post-lab entry of observations will lower your grade. Please note that lab notebooks will be picked up periodically during the semester for grading by the TA. Late notebooks WILL LOSE 1 LETTER GRADE PER DAY and WILL NOT BE ACCEPTED AFTER December 16Feedback: Students are highly encouraged to turn in your writing assignment early to get feedback so that you can revise the document before you turn it in for grading.Laboratory Notebook Requirements:Each weekly experiment in your lab notebook needs to be fully documented and should read like a formal lab paper. These are the required items and their point values for each week’s lab period:Table of ContentsExample:Table of ContentsBIOB 4 1 1 -  Im m u n o l o g y  L a b o r a t o r y  F a l l  2 0 1 4 3Lab 2 -  Cells and Organs of the Immune System I page 3Lab 3 -  Cells and Organs of the Immune System II page 7etc.In your lab notebook you must have these sections:Introduction -  (1pt) -  a couple of sentences/paragraphs describing what the lab will cover.Procedure -  (1 pt)Example:BIOB 411, Immunology Lab, Professor Wetzel, Fall 2011, University of Montana, pages 11-12**Note any deviations from lab manual procedure**Results -  (1 pt) -  Numbers, drawings, etc. This is a description of your data and includes any calculations of % difference, etc. that isn’t in your figures.Discussion/Conclusion -  (1 pt) -  What was expected and what did it mean? Did everything turn out as expected? Was there error? Why did the error occur? Observations, Ideas, Thoughts, Etc.Take Home Problems - (1 pt) -  These will be on the chalkboard.

BIOB 411.01: Immunology Laboratory

https://scholarworks.umt.edu/cgi/viewcontent.cgi?article=2559&amp;context=syllabi

BIOB 411.01: Immunology Laboratory

Abstract

Similar works

Full text

Available Versions

University of Montana