Abel

Alavi

Aldridge

Allgaier

Ausmees

Belas

Berger

Brinkhoff

Bruhn

Buchan

Charlson

Christen

Cotter

D'Alvise

Ditta

Duerig

Geng

Gjermansen

Gonzalez

Gram

Hengge

Hjelm

Holcapek

Huitema

Keen

Kovach

Lambertsen

Magdenoska

Matilla

McDougald

Merritt

Miller

Moran

Newell

Newton

O'Toole

Paul

Planas

Porsby

Punta

Ruiz-Ponte

Ryjenkov

Sambrook

Schell

Schmidt

Shiba

Simm

Slightom

Sorokin

Spangler

Srivastava

Sule

Tamayo

Tamura

Thompson

Viollier

Waters

Weinhouse

Werner

Wietz

Witters

Wolfe

Paul W. D'Alvise

Olivera Magdenoska

Jette Melchiorsen

Kristian F. Nielsen

Lone Gram

Crossref

Environmental Microbiology

Biofilm formation and antibiotic production in<i>Ruegeria mobilis</i>are influenced by intracellular concentrations of cyclic dimeric guanosinmonophosphate

In many species of the marine Roseobacter clade, periods of attached life, in association with phytoplankton or particles, are interspersed with planktonic phases. The purpose of this study was to determine whether shifts between motile and sessile life in the globally abundant Roseobacter clade species Ruegeria mobilis are associated with intracellular concentrations of the signal compound cyclic dimeric guanosinmonophosphate (c-di-GMP), which in bacteria regulates transitions between motile and sessile life stages. Genes for diguanylate cyclases and phosphodiesterases, which are involved in c-di-GMP signalling, were found in the genome of R. mobilis strain F1926. Ion pair chromatography-tandem mass spectrometry revealed 20-fold higher c-di-GMP concentrations per cell in biofilm-containing cultures than in planktonic cells. An introduced diguanylate cyclase gene increased c-di-GMP and enhanced biofilm formation and production of the potent antibiotic tropodithietic acid (TDA). An introduced phosphodiesterase gene decreased c-di-GMP and reduced biofilm formation and TDA production. tdaC, a key gene for TDA biosynthesis, was expressed only in attached or biofilm-forming cells, and expression was induced immediately after initial attachment. In conclusion, c-di-GMP signalling controls biofilm formation and biofilm-associated traits in R. mobilis and, as suggested by presence of GGDEF and EAL domain protein genes, also in other Roseobacter clade species

D'Alvise, Paul

Magdenoska, Olivera

Melchiorsen, Jette

Nielsen, Kristian Fog

Gram, Lone

Online Research Database In Technology

Biofilm formation and antibiotic production in <em>Ruegeria mobilis</em> are influenced by intracellular concentrations of cyclic dimeric guanosinmonophosphate

http://orbit.dtu.dk/ws/files/70017278/emi12265.pdf

Biofilm formation and antibiotic production in <em>Ruegeria mobilis</em> are influenced by intracellular concentrations of cyclic dimeric guanosinmonophosphate

Abstract

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Crossref

Online Research Database In Technology