Ana Rute Neves

Binder

Bland

Chua

Clere

Gredell

Gresele

Halls

Hanahan

Hirooka

Hwang

Jérôme Maury

Kaneko

Kape

Kumar

Leonard

Marienhagen

Marin

Miller

Moreira

Mustafi

Prescott

Rossen

Sailesh Malla

Sambrook

Santos

Silva-Rocha

Solvej Siedler

Steen G. Stahlhut

Tang

Tropel

van Sint Fiet

Ververidis

Zaat

Crossref

Metabolic Engineering

Novel biosensors based on flavonoid-responsive transcriptional regulators introduced into Escherichia coli

This study describes the construction of two flavonoid biosensors, which can be applied for metabolic engineering of Escherichia coli strains. The biosensors are based on transcriptional regulators combined with autofluorescent proteins. The transcriptional activator FdeR from Herbaspirillum seropedicae SmR1 responds to naringenin, while the repressor QdoR from Bacillus subtilis is inactivated by quercetin and kaempferol. Both biosensors showed over a 7-fold increase of the fluorescent signal after addition of their specific effectors, and a linear correlation between the fluorescence intensity and externally added flavonoid concentration. The QdoR-biosensor was successfully applied for detection of kaempferol production in vivo at the single cell level by fluorescence-activated cell sorting. Furthermore, the amount of kaempferol produced highly correlated with the specific fluorescence of E. coli cells containing a flavonol synthase from Arabidopsis thaliana (fls1). We expect the designed biosensors to be applied for isolation of genes involved in flavonoid biosynthetic pathways. © 2013 The Authors

Siedler, Solvej

Stahlhut, Steen Gustav

Malla, Sailesh

Maury, Jerome

Neves, Ana Rute

Online Research Database In Technology

http://orbit.dtu.dk/ws/files/68547774/1_s2.0_S1096717613001031_main.pdf

Novel biosensors based on flavonoid-responsive transcriptional regulators introduced into Escherichia coli

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Online Research Database In Technology