Habekuß, A.

Himmelbach, A.

Mascher, M.

Nöh, C.

Ruge-Wehling, B.

Scholz, U.

Schweizer, G. F.

Stein, N.

Wendler, N.

English

JKI Open Journal Systems (Julius Kühn-Institut)

Nachwuchswissenschaftlerforum / Young Scientists Meeting 2012 Nöh et al. 43 Berichte aus dem Julius Kühn-Institut 167. 2012 Fine mapping of 5 resistance genes on introgressions of Hordeum bulbosum in barley with SNP markers  Nöh, C.1, Wendler, N.2, Mascher, M.2, Scholz, U.2, Himmelbach, A.2, Stein, N.2, Habekuß, A.3,  Schweizer, G.F.4, Ruge-Wehling, B.1  1 Julius Kühn-Institute, Institute for Breeding Research on Agricultural Crops, Groß Lüsewitz 2 Leibniz Institute of Plant Genetics and Crop Plant Research, Gaterleben 3 Julius Kühn-Institute, Institute for Resistance Research and Stress Tolerance, Quedlinburg 4 Bayerische Landesanstalt für Landwirtschaft, Institut für Pflanzenbau und Pflanzenzüchtung, Freising Email of corresponding author: christiane.noeh@jki.bund.de  Barley growing is threatened by plant diseases such as scald (Rhynchosporium secalis), leaf rust (Puccinia hordei) or the soil-borne virus complex of BaMMV, BaYMV-1 and -2, the latter of which is transmitted by the fungus Polymyxa graminis. Breeding of resistant varieties can minimize the yield losses. The wild Hordeum species H. bulbosum, which makes up the secondary gene pool of barley, is a source of resistance genes against these fungi and viruses. Interspecific crosses of H. vulgare x H. bulbosum resulted in different recombinant diploid barley progeny with dominantly inherited immunity against the two fungi and the virus complex, respectively. Two virus resistance genes, Rym14Hb and Rym16Hb, have been introgressed to the short arm of chromosome 6 (6HS) and the long arm of chromosome 2 (2HL), respectively. The gene Rrs16Hb, which confers immunity to R. secalis, was introgressed to the short arm of barley chromosome 4 (4HS). Two introgressions containing genes for resistance to P. hordei are present on chromosomes 2HL and 5HL, respectively. Mapping populations for the 5 resistance genes segregated in a 3:1 ratio of resistant to susceptible plants, suggesting a dominant monogenic inheritance of the different resistances. For the fine mapping of these genes SNP markers will be developed on the basis of sequence information generated by exome capture and the mapping populations genotyped with the Illumina VeraCode GoldenGate Genotyping Assay, Genotyping By Sequencing and Pyrosequencing. Finally,  recombinant plants with reduced introgression sizes containing the resistance genes will be identified by marker-assisted selection for barley breeding.  

Fine mapping of 5 resistance genes on introgressions of Hordeum bulbosum in barley with SNP markers

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Fine mapping of 5 resistance genes on introgressions of Hordeum bulbosum in barley with SNP markers

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