Graner, Andreas

Habekuß, Antje

Ordon, Frank

Perner, Katja

Perovic, Dragan

English

JKI Open Journal Systems (Julius Kühn-Institut)

Nachwuchswissenschaftlerforum / Young Scientists Meeting 2015 Perner et al. Fine mapping and identification of candidate genes for a BaYMV/BaYMV-2 resistance gene located on chromosome 5H of barley  Katja Perner 1, 2, Dragan Perovic 2, Antje Habekuß 2, Andreas Graner1, Frank Ordon2 1 Leibniz-Institute of Plant Genetics and Crop Plant Research, Gatersleben 2Julius Kühn-Institute, Institute for Resistance Research and Stress Tolerance, Quedlinburg Email of corresponding author: katja.perner@jki.bund.de One of the most important diseases of winter barley in Europe and East Asia is barley yellow mosaic virus disease caused by different strains of Barley yellow mosaic virus (BaYMV) and Bar-ley mild mosaic virus (BaMMV) leading to yield losses up to 50% in susceptible winter barley varieties. Due to the transmission by the soil-borne plasmodiophorid Polymyxa graminis, chemical measures to prevent high yield losses are neither effective nor ecologically sound. Thus, breeding for resistance is of prime importance in order to ensure winter barley produc-tion in the growing area of infested fields. Up to now, nine different loci conferring resistance to the different strains of BaMMV and BaYMV are known. In order to get detailed infor-mation on the structure and function of a resistance gene being only effec-tive against BaYMV and BaYMV-2 lo-cated in the centromeric region of chromosome 5H a map based cloning approach was conducted. For marker saturation of the target interval all available sequence information in bar-ley and the synteny to rice, Sorghum, Brachypodium and sequence infor-mation included in the genome zipperwas used. Phenotyping for BaYMV/BaYMV-2 resistance of respec-tive segmental RILs derived from a high resolution mapping population comprising 5000 F2-plants was carried out in field trials followed by DAS-ELISA. Based on marker saturation and phenotyping of 691 RILs the resistance gene was mapped in an interval of 0.22% recombination. By an additional exome capture sequencing approach of the parental lines, 249 morex contigs containing 256 genes were located in this interval. Out of these, two candidate genes were identified of which one is co-segregating with the resistance locus. Sequence analy-sis of this gene revealed 3 functional SNPs and a 6 bp deletion in the resis-tant parent. Further analyses are in progress to get information whether this gene confers resistance to BaYMV/BaYMV-2. This shows that combination of different barley genomic resources and new generation sequencing technologies, applied in map based cloning proce-dures, is a powerful tool to accelerate soil-borne virus resistance gene isola-tion in barley.  Berichte aus dem Julius Kühn-Institut 181 13

Fine mapping and identification of candidate genes for a BaYMV/BaYMV-2 resistance gene located on chromosome 5H of barley

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Fine mapping and identification of candidate genes for a BaYMV/BaYMV-2 resistance gene located on chromosome 5H of barley

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