Chandrapatya, A.

Fields, P. G.

Suthisut, D.

English

JKI Open Journal Systems (Julius Kühn-Institut)

 10th International Working Conference on Stored Product Protection 746  Julius-Kühn-Archiv, 425, 2010 Biological activity of essential oils of Alpinia conchigera rhizome against Sitophilus zeamais and Tribolium castaneum Suthisut, D. *2, Fields, P.G. #1, Chandrapatya, A.2 1 Cereal Research Centre, Agriculture & Agri-Food Canada, 195 Dafoe Road, Winnipeg, Manitoba, R3T 2M9, Canada, paul.fields@agr.gc.ca 2 Department of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok, Thailand, 10900, dsuthisut@yahoo.com, chandrapatya@yahoo.com * Corresponding author # Presenting author DOI: 10.5073/jka.2010.425.098 Abstract Research dealing with plant products is a new direction as an alternative to conventional insecticides for stored-product insect control (Shaaya et al., 1991, 1997). Alpinia conchigera Griffin (Zingiberaceae) is a native plant in southern Thailand, and it has a wide variety of the essential oils (Ibrahim et al., 2009). The toxicity and repellency of the water distilled essential oils from rhizome of A. conchigera was evaluated against the major stored-product insect pests, maize weevil, Sitophilus zeamais Motschulsky and red flour beetle, Tribolium castaneum (Herbst) 1-14 day-old adults at 29+2 oC and 65+5% r.h. In fumigation trials (Liu and Ho, 1999), the mortality was assessed at concentrations ranging from 74 to 667 µL/L in air with exposure times ranging from 3 to 24 h. There was complete mortality of S. zeamais at 222 µL/L after 24 h, whereas 593 µL/L for 24 h was required for complete mortality of T. castaneum. Sitophilus zeamais adults (LC50, fiducial limits: 121, 114-129 µL/L) were more susceptible to essential oils of A. conchigera than T. castaneum (295, 203-369 µL/L) (Table 1). Contact toxicity was assayed by topical application to insect thoraxes (Liu and Ho, 1999) at different concentrations (10 to 40%). Sitophilus zeamais adults (LC50, 27, 18-40 µg/mg) had the same mortality as T. castaneum (LC50, 34, 28-47 µg/mg) (Table 2). A filter paper choice bioassay (Ko et al., 2009) of essential oils of A. conchigera in 100% ethanol showed that T. castaneum has repelled more than S. zeamais (Table 3). Keywords: Alpinia conchigera, Sitophilus zeamais, Tribolium castaneum, Essential oils, Toxicity Table 1 Fumigation toxicity of essential oils from Alpinia conchigera  rhizome against Sitophilus zeamais and Tribolium castaneum at 29 oC after 24 h. Insect LC50 (µL/L) 95% confidence Intervals (µL/L) LC95 (µL/L) 95% confidence Intervals (µL/L) Degrees of freedom Chi-square S. zeamais 121 113-128 180 168-196 8      0.395 T. castaneum 294 203-368 417 350-658 8 170.09  Table 2 Contact toxicity of Alpinia conchigera rhizome essential oils against Sitophilus zeamais and Tribolium castaneum at 29 oC after 24 h. Insect LC50 (µg/mg) 95% confidence Intervals (µg/mg) LC95 (µg/mg) 95% confidence Intervals (µg/mg) Degrees of freedom Chi-square S. zeamais 26 18-39 51 38-103 3 18.04 T. castaneum 34 28-46 60 47-101 3 9.33  10th International Working Conference on Stored Product Protection Julius-Kühn-Archiv, 425, 2010  747 Table 3 Percent repellency (PR) of Alpinia conchigera rhizome essential oils against Sitophilus zeamais and Tribolium castaneum using treated filter paper test* PR (Mean% + SD) Insect Oil (µg/cm2) Time after insect release (h)   1 2 3 4 5 PR (Mean%) S. zeamais 0.16 32+59 b     36+59 b    56+38 b     68+41 a     60+47 a 50  0.31 88+11 a       68+61 ab      76+26 ab     80+14 a     60+20 a 74  0.47   96+9 a   96+9 a    88+18 a     72+30 a     72+33 a 85  0.63 100+0 a 100+0 a   96+9 a     72+30 a     48+39 a 83  0.79 100+0 a   96+9 a   96+9 a     80+45 a     60+14 a 86 T. castaneum 0.16 80+20 a 100+0 a     92+11 a     76+26 b     52+30 b 80  0.31 72+18 a     80+25 b     92+11 a       80+14 ab     84+22 a 82  0.47 84+17 a     96+9 ab     92+11 a       92+11 ab 100+0 a 93  0.63 92+18 a 100+0 a   96+9 a     96+9 ab        80+28 ab 93  0.79   96+9 a     96+9 ab   96+9 a 100+0 a      88+18 a 95 *Five replicates of 10 insects in each replication, for each insect, means in same column followed by the different letters are significantly (P>0.05) Duncan’s multiple range test (DMRT).  References Ko, K., Juntarajumnong, W., Chandrapatya, A., 2009. Repellency, fumigant and contact toxicities of Litsea cubeba (Lour.) Persoon against Sitophilus zeamais Motschulsky and Tribolium castaneum (Herbst). Kasetsart Journal Natural Sciences 43, 56-63. Liu, Z.L., Ho, S.H., 1999. Bioactivity of the essential oil extracted from Evodia rutaecarpa Hook F. et Thomas against the grain storage insects, Sitophilus zeamais Motsch and Tribolium castaneum (Herbst). Journal of Stored Products Research 35, 317-328. Ibrahim, H., Aziz, A.N., Syamsir, D.R.,  Ali, N.A.M.,  Mohtar, M., Ali, R.M., Awang, K., 2009. Essential oils of Alpinia conchigera Griff. and their antimicrobial activities. Food Chemistry 113, 575-577. Shaaya, E., Kostjukovski, M., Eilberg, J., Sukprakarn, C., 1997.  Plant oils as fumigants and contact insecticides for the control of stored-product insects. Journal of Stored Products Research 33, 7-15. Shaaya, E., Ravid, U., Paster, N., Juven, B., Zisman, U., Pisarrev, V., 1991. Fumigant toxicity of essential oils against four major stored-product insects. Journal of Chemical Ecology 17, 499–504.   

Biological activity of essential oils of Alpinia conchigera rhizome against Sitophilus zeamais and Tribolium castaneum

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Biological activity of essential oils of Alpinia conchigera rhizome against Sitophilus zeamais and Tribolium castaneum

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