The file contains 213,916 GBS-SNPs obtained using genotyping-by-sequencing on the Senegalese sorghum accessions in the USDA-NPGS GRIN. Single-end sequence reads obtained from Illumina sequencing HiSeq 2500 were processed with the TASSEL 5 GBS v2 pipeline and sequence tags were aligned to the sorghum reference genome, BTx623 for SNP discovery. Only SNPs with 0.01, and biallelic SNPs are present in this data set