Karakteristik Morfologi dan Biokimiawi Isolat Selulolitik Cytophaga sp

Abstract

This study aimed to know the characteristic morphology and biochemical of cellulolytic isolate Cytophaga sp which isolated from bovine rumen fluid waste. Conducted growing isolates cellulolytic bacteria in selective media liquid consist of MgSO4.7H2O 0,05 g/100 ml, Na2HPO4.2H2O 0,5 g/100 ml, NaCl 0,23 g/100 ml, yeast extract 0,2 g/100 ml, CMC 1 g/100 ml in Erlenmeyer.and then 1 ose bacteria colonies grown in solution liquid medium then incubated for 24 hours at temperature 37°C. Research carried out by descriptive method, consists of three stages. In the first done isolation and purification isolates bacteria from a liquid rumen. The second stage was screening cellulolytic bacteria in semikuantitatif in a media CMC plate .The third stage was characterization morphology and biochemical test. The result of morphological and biochemical test indicated that cellulolytic isolate was Cytophaga sp. Keywords: cellulolytic bacteria, morphological, biochemical test, liquid bovine rume

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