Kopchuk, T.G.

Цифровий Репозиторій - Інтелектуальні Фонди Буковинського державного медичного університету

383points at the nephroprotective effect of KATP channels activation with Flocalin predominantly inthe proximal part of the nephron.Horoshko O.M.CHRONORHYTHMOLOGIC FEATURES OF LIPIN ON ANTIOXIDANT PROTECTIONINDICATORS IN RATS WITH MODEL PATHOLOGIESDepartment of Pharmaceutical Botany and PharmacognosyBukovinian State Medical UniversityAny biological system of the organism is subjected to the natural functioning organization.Renal function has a conspicuous circadian periodicity. Thus, circadian rhythms of biochemicalparameters in organs and tissues are indicators of the body state, including kidneys. Manypathological processes are accompanied by a violation of the temporal organization of physiologicalfunctions, which is also characteristic of the pathogenesis of the acute renal failure development.The aim of the given study was to establish chronorhythmic indicator changes of antioxidantprotection of renal tissues under the conditions of model pathology with a single injection of lipin.The experiments were conducted on 21 adult outbred white rats, weighing 120-160 g. Acuterenal failure was caused by intramuscular administration of 50% glycerol solution at a dose of 10mg/kg. Lipin was administered at a dose of 500 mg/kg once intraperitoneally in 40 min afteradministration of glycerol. To perform biochemical studies, kidney tissue was collected afterdecapitation of rats for the 12th hour of the experiment with a 6-hour interval: 4 times a day - at 8am, 2 pm, 8 pm and 2 am.Antioxidant effects were evaluated by the content of lipid peroxidation products(malondialdehyde (MDA)) and proteins (protein oxidation products (POP)).The obtained data on MDA content in the animal kidney tissues with model pathologyreached a minimum value at 8 pm and a maximum one at 2 am, which was 1.6 times higher thancontrol group and remained high at 8 am. Lipin reduced the MDA content on the background ofacute renal failure by 1.3 times during the period of its maximum value by 2 hours, and at 8 am theeffect of the drug reduced the MDA content by 1.2 times. The POP content reached its peak in theaffected animals at 8 pm (1.3 times) compared with the control group. Lipin with a single injectionhad the greatest effect (in 1.4 times) on the intensity of the POP formation at 8 pm.Thus, in animals with model pathology there were changes in the structure and nature ofcircadian rhythms that characterized antioxidant protection. The correction of model pathology bylipin should be noted to enhance since 8 pm till the end of the experiment.Therefore, the treatment of acute renal failure should be prescribed taking into account therhythm of antioxidant protection processes and the use of antioxidant drugs is recommended mainlyin the afternoon.Kopchuk T.G.HISTOLOGICAL CHANGES IN THE KIDNEY STRUCTURE IN THE DYNAMICS OFFEVER DEVELOPMENTDepartment of PharmacologyBukovinian State Medical UniversityFever is a typical pathological process characterized by a shift of the thermoregulatoryreference point to a higher level of body temperature regulation due to the influence of pyrogenicsubstances. Fever development includes three stages: rising of temperature, maintenance of hightemperature and decrease in body temperature.The aim of our experiment was to study the histological changes the kidney structure in thedynamics of fever development and detect the mechanisms of damage to nephrocytes of the kidneycortex, medulla and papilla in conditions of the fever development.Research was conducted on 60 non-linear white male rate weighting 130-180 g, maintainedunder the standard vivarium conditions with a constant temperature and humidity. Aseptic feverwas induced according to recommendations by a single subcutaneous injection of pyrogenal at a384dose of 25 μg/kg. During development of the fever, rectal temperature was measured every 30 min.Animals were withdrawn from the experiment 24 h later, while blood, urine and kidneys weresampled for biochemical and histopathological assessments. Statistical processing of the obtaineddata was performed using the SPSS Statistics 17.0 software.According to obtained results, in the first stage of fever, heat production predominates overheat transfer, which in pathogenesis is actually the effect of low temperature and is accompanied bythe activation of the sympathetic and renin-angiotensin systems, respectively. This explains thedecrease in GFR. The increase in protein excretion in the urine in the first stage of fever is due tothe ischemic effect of angiotensin 2 on the cortical region of the kidneys, where localized proximaltubules responsible for protein reabsorption. Fibrin filaments were deposited at the site of destroyednephrocytes, which was detected during Slinchenko staining as a small-focal character of changesin the properties of proteins with a shift in color to red. In the second stage of fever, the bodytemperature reaches the level of a new reference point, heat production is balanced with heattransfer, and fever performs its biological role. This normalizes the activity of the renin-angiotensinsystem, which was increased in the first stage of fever, resulting in increased GFR and there is anexpansion of the capsule Shumlyansky-Bowman. Inhibition of distal reabsorption of sodium ionswith dystrophic changes in the epithelium of the tubules of this nephron is due to energy deficiencyof the kidneys, because the energy of ATP in the second stage of fever is used to ensure itsbiological role. Inhibition of proximal reabsorption of sodium ions and insignificant dystrophicchanges of the epithelium of the proximal tubules in the third stage of fever decrease in temperaturewith predominance of heat transfer over heat production due to hypoxia of the kidneys due to bloodclotting due to intense sweating.With the development of aseptic fever on white nonlinear male rats with hyponatrium dietfound: in the first stage (temperature rise) vacuolar dystrophy of the epithelium of the proximaltubules and small-focal nature of changes in the properties of proteins with a shift in color to red(standing) high level) expansion of the lumen of the Shumlyansky-Bowman capsule and dystrophicchanges in the epithelium of the distal tubules, in the third stage (decrease in temperature) moderateexpansion of the lumen of the Shumlyansky-Bowman capsule and insignificant dystrophic changesin the epithelium of the proximal tubules. Morphological disorders in the dynamics of thedevelopment of fever reflect the nature of changes in renal function.Korovenkova O.M.THE EFFECT OF THIOCETAM ON THE EXCRETORY FUNCTION OF THE KIDNEYSUNDER THE CONDITIONS OF BLOCKADE OF PROSTAGLANDIN SYNTHESISDepartment of PharmacyBukovinian State Medical UniversityIt is known that prostaglandins (PG),  namely, eicosanoids, that synthesized in kidney cellsas natriuretic (PG E) and  sodium delays (GHG F2a), take a direct part in the regulation ofrenal circulation  and kidney function.The purpose of the paper was to conduct a series of experiments against the background ofinhibition of prostaglandin.We studied 18 male rats, which were divided into 3 groups: with and without thiocetam 250mg/kg (control group) and with indometacyn 100mg/kg. An experimental and case-control designwas used. Biochemical methods were used in the study of blood and urine. Renal sodium transportwas calculated after 2 hours, taking into account body weight. Kidney function was estimated at 5%including by weight of water.According to our results, the inhibition of prostaglandins by indomethacin in rats reducesdiuresis by 30%, inhibition of prostaglandins by indomethacin in rats reduces glomerular filtrationrate by 1.7 times, the concentration of sodium ions in blood plasma was decreased by 30%, plasmacreatinine concentration was also decreased (from71,3+2.83μmol/l to 61.8+1.25μmol/l, P<0,01), aswell as decreased renal excretion (from 2.4+0.09μmol/2h in control up to 1.2+0.04 μmol/2h in theexperiment, P <0,01).

Histological changes in the kidney structure in the dynamics of fever development

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Histological changes in the kidney structure in the dynamics of fever development

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Цифровий Репозиторій - Інтелектуальні Фонди Буковинського державного медичного університету