Bagwell, C.E.

Carroll, S.L.

Criddle, C.S.

Fields, M.W.

Hazen, T.C.

Jaridne, P.M.

Liu, X.

Yan, T.

Zhou, J.

eScholarship - University of California

Lawrence Berkeley National LaboratoryRecent WorkTitlePolyphasic characterization of microbial communities under the stressful conditions of nitrate, heavy metals, radionuclides, and acidic pH in contaminated groundwaterPermalinkhttps://escholarship.org/uc/item/6hm6w8t8AuthorsFields, M.W.Yan, T.Liu, X.et al.Publication Date2004-03-12eScholarship.org Powered by the California Digital LibraryUniversity of CaliforniaPolyphasic characterization of microbial communities under the stressful conditions of nitrate, heavy metals, radionuclides, and acidic pH in contaminated groundwater   M.W. Fields1, T. Yan2, X. Liu2, C.E. Bagwell3, S.L. Carroll3, P.M. Jaridne2, C.S.Criddle4, T.C. Hazen5, and J. Zhou2  Miami University1, Oak Ridge National Laboratory2, Savannah River Technology Center3, Stanford University4, and Lawarence Berkeley National Laboratory5  Clonal libraries of multiple genes (SSU rRNA gene, nirK, nirS, amoA, pmoA, and dsrAB; n=6100) were constructed from groundwater samples (n=6) that varied in degrees of contamination.  The sites were in proximity to a concentration plume, and the following geochemical parameters were measured:  pH, nitrate, uranium, nickel, sulfate, and TOC (total organic carbon).  The nir genes encode nitrite reductase, amo/pmoA encode ammonia monooxygenase, and dsrAB encode sulfite reductase.  Principle components analysis (PCA) was used to compare the relationships between the sites with respect to the unique operational taxonomic unit (OTU) distributions within the different clonal libraries.  When geochemical characteristics were analyzed with PCA, the data suggested that the samples varied significantly with respect to nitrate and sulfate.  The sites were grouped differently when compared upon the basis of the SSU rDNA genes or each of the functional genes.  The diversity of amoA genes was much lower compared to the other functional genes, and the one sample with high nitrate and circum neutral pH appeared to be different from the other sites.  When all gene OTUs were used in the analyses, the sites were more similar than in any other comparison, and the background site was grouped with the acidic, contaminated sites.  The results indicated that a combination of the contaminants (i.e., nitrate, uranium, nickel) and pH levels have impacted the bacterial communities at the respective sites in different and dynamic ways.  These data suggest that even though the background site was phylogenetically distinct from the acidic sites, that the extreme conditions of the acidic samples might be more analogous to the limiting nutrient conditions of the background site.   

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Polyphasic characterization of microbial communities under the stressful conditions of nitrate, heavy metals, radionuclides, and acidic pH in contaminated groundwater

https://escholarship.org/uc/item/6hm6w8t8

Polyphasic characterization of microbial communities under the stressful conditions of nitrate, heavy metals, radionuclides, and acidic pH in contaminated groundwater

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