Binbin Cao

Chonglv Feng

Damin Luo

Meks Tukayo

Wenzhen Fang

Xuri Zhang

Yinan Wang

Ying Long

YU. Liang

Archives of Biological Sciences

Although the third-stage larvae of Angiostrongylus cantonensis (AcL3) are
   thought to initiate infection by penetrating the epithelium of the small
   intestine, the mode of intestinal invasion remains obscure. Considering the
   inaccessibility of the gut tract and the need to sacrifice animals for this
   type of study, we devised an in vitro cell-parasite co-culture system to
   examine the initial cellular and molecular events between AcL3 and host
   epithelia. No apoptosis augmentation was detected in enterocytes after
   introduction of larvae. A significant increase in dead cells was detected in
   IEC-6, NCM460 and 293T after incubating for 4 h, with AcL3 wounding rat small
   intestinal epithelial cells IEC-6 more rapidly. Under a scanning electron
   microscope (SEM), cell gap opening was visualized in the IEC-6 monolayer
   treated with AcL3. Loosening of the extracellular matrix (ECM) of the
   monolayer was found to be involved in the parasite-cell interactions.
   Pretreating the AcL3 with a protease inhibitor attenuated its penetration
   ability of the artificial intestine barrier. In conclusion, AcL3 broke
   through the intestinal barrier of the host with the assistance of mechanical
   injury and the opening of a cell gap, but without causing apoptosis. The
   interaction platform presented here may provide direct insight into the
   cellular and molecular events during worm invasion of host enterocytes.</jats:p

Crossref

Initial events in the breakthrough of the epithelial barrier of the small intestine by Angiostrongylus cantonensis

Initial events in the breakthrough of the epithelial barrier of the small intestine by Angiostrongylus cantonensis

Abstract

Similar works

Full text

Available Versions

Crossref