A Caruso

A Zdanov

AE Ryan

BJ Lange-Brokaar de

BO Diekman

C Sullivan

CI Westacott

CM Coleman

DF Fiorentino

E Schurgers

F Apparailly

F Pessler

F Rousset

GD Palmer

GJ Osch van

GM Buul van

H Hulejova

H Ishii

H Nakamura

JG Evans

JJ Choi

JL Hsieh

JM Murphy

JU Yoo

K Johnson

K Nemeth

KP Pritzker

L Thompson-Snipes

L Ziegler-Heitbrock

LI Sakkas

M Horie

M Huurne ter

M Song

MC Honorati

MM Duffy

NF Go

P Vieira

P Wojdasiewicz

PC Shen

PH Hart

PL Lent van

RD Malefyt

RF Loeser

S Alsalameh

SH Yang

YK Jung

Abstract
                Background
                Recent efforts in osteoarthritis (OA) research have highlighted synovial inflammation and involvement of immune cells in disease onset and progression. We sought to establish the in-vivo immune response in collagenase-induced OA and investigate the ability of human mesenchymal stem cells (hMSCs) overexpressing viral interleukin 10 (vIL-10) to modulate immune populations and delay/prevent disease progression.
              
                Methods
                Eight-week-old male C57BL/6 mice were injected with 1 U type VII collagenase over two consecutive days. At day 7, 20,000 hMSCs overexpressing vIL-10 were injected into the affected knee. Control groups comprised of vehicle, 20,000 untransduced or adNull-transduced MSCs or virus alone. Six weeks later knees were harvested for histological analysis and popliteal and inguinal lymph nodes for flow cytometric analysis.
              
                Results
                At this time there was no significant difference in knee OA scores between any of the groups. A trend toward more damage in animals treated with hMSCs was observed. Interestingly there was a significant reduction in the amount of activated CD4 and CD8 T cells in the vIL-10-expressing hMSC group.
              
                Conclusions
                vIL-10-overexpressing hMSCs can induce long-term reduction in activated T cells in draining lymph nodes of mice with collagenase-induced OA. This could lead to reduced OA severity or disease progression over the long term.
              </jats:sec

Eric Farrell

Niamh Fahy

Aideen E Ryan

Cathal O Flatharta

Lisa O’Flynn

Thomas Ritter

J Mary Murphy

Crossref

Stem Cell Research & Therapy

vIL-10-overexpressing human MSCs modulate naïve and activated T lymphocytes following induction of collagenase-induced osteoarthritis

https://stemcellres.biomedcentral.com/track/pdf/10.1186/s13287-016-0331-2?site=stemcellres.biomedcentral.com

vIL-10-overexpressing human MSCs modulate naïve and activated T lymphocytes following induction of collagenase-induced osteoarthritis

Abstract

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