The <i>maf</i> locus drives EGFP in putative neurosecretory brain cells

Abstract

<p>. (<b>A</b>) Scheme of donor vector pTol2{maf::egfp}^frkt1208 containing a 3.6 kB upstream of the <i>maf</i> start codon (compare to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0093076#pone-0093076-g001" target="_blank">Figure 1A</a>). (<b>B</b>) Expression of endogenous <i>maf</i> RNA (arrowhead) in the medial central brain of early metatrochophore larvae. (<b>C</b>) Synexpression of the <i>insulin-like peptide 2</i>/<i>ilp2</i> mRNA in the same region (arrowhead), suggesting that <i>maf</i> demarcates neurosecretory cells. (D–F) EGFP expression driven by pTol2{maf::egfp}^frkt1208 demarcates cells in the same region as endogenous <i>maf</i>.</p