Determining Gene Specific Chromatin Differences in \u3ci\u3eSulfolobus solfataricus\u3c/i\u3e: Expression of MerR Protein for Targeted-ChIP Antibody Production
In this project the repressor protein MerR from the Sulfolobus solfataricus mercury resistance operon was cloned into pET28b and transformed into Roetta 2 E.coli strains for overexpression and purification. Large quantities of recombinant MerR will be used for subsequent injection into a mammalian host for antibody production. These antibodies will be used in targeted-ChIP studies in which gene specific chromatin modification states will be analyzed. The overproduction of MerR is part of a larger project where future research could produce data on whether gene expression levels and chromatin modifiation states could be correlated at an individual gene level, possibly suggesting a novel epigenetic mechanism in Archaea
