Validation of the bespoke surface fluorescence apparatus.

Abstract

<p><b>A,</b> Time course of NAD(P)H (top) and flavoprotein (bottom) surface fluorescence and the reflectance signals at the corresponding excitation and emission wavelengths together with data for the left ventricular pressure (LVP). <b>B,</b> Example of Indo-1 fluorescence transients recorded at 405 nm (in blue) and at 485 nm (in red), and Indo-1 ratio calculated after subtracting background autofluorescence. The top trace shows corresponding LVP data.</p

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