Abstract

<p>Methicillin-induced filaments expressing FtsZ-Ve (NZ3900 [pGIBLD031]) (A) or Ve-PBP2b (NZ3900 [pGIBLD031]) (B) were transferred to methicillin-free agar pads to study their localization dynamics by time-lapse microscopy (phase contrast (PC) and fluorescence) during filament reversion (see also <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0198014#pone.0198014.s019" target="_blank">S5</a>–<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0198014#pone.0198014.s024" target="_blank">S10</a> Movies). Localization of the protein at the constricting septum and future division sites is highlighted with red and orange arrows, respectively. Only the early steps of filament reversion are highlighted. Scale bars, 2 μm. (C) Schematic diagrams summarizing localization patterns of FtsZ-Ve (green) and Ve-PBP2b (yellow).</p

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