Also, variously referred to as: House bills; House documents; House legislative documents; legislative documents; General Court documents

Chaonan Shi (3419669)

Dangqun Cui (479000)

Feng Chen (1105700)

Lei Zhao (144153)

Lingran Zhang (5285855)

Ning Zhang (23771)

Crops
are often subjected to a combination of stresses in the field.
To date, studies on the physiological and molecular responses of common
wheat to a combination of osmotic and cold stresses, however, remain
unknown. In this study, wheat seedlings exposed to osmotic-cold stress
for 24 h showed inhibited growth, as well as increased lipid peroxidation,
relative electrolyte leakage, and soluble sugar contents. iTRAQ-based
quantitative proteome method was employed to determine the proteomic
profiles of the roots and leaves of wheat seedlings exposed to osmotic-cold
stress conditions. A total of 250 and 258 proteins with significantly
altered abundance in the roots and leaves were identified, respectively,
and the majority of these proteins displayed differential abundance,
thereby revealing organ-specific differences in adaptation to osmotic-cold
stress. Yeast two hybrid assay examined five pairs of stress/defense-related
protein–protein interactions in the predicted protein interaction
network. Furthermore, quantitative real-time PCR analysis indicated
that abiotic stresses increased the expression of three candidate
protein genes, i.e., TaGRP2, CDCP, and Wcor410c in wheat leaves. Virus-induced
gene silencing indicated that three genes TaGRP2, CDCP, and Wcor410c
were involved in modulating osmotic-cold stress in common wheat. Our
study provides useful information for the elucidation of molecular
and genetics bases of osmotic-cold combined stress in bread wheat

FigShare

Identification
of Proteins Using iTRAQ and Virus-Induced
Gene Silencing Reveals Three Bread Wheat Proteins Involved in the
Response to Combined Osmotic-Cold Stress

https://figshare.com/articles/Identification_of_Proteins_Using_iTRAQ_and_Virus-Induced_Gene_Silencing_Reveals_Three_Bread_Wheat_Proteins_Involved_in_the_Response_to_Combined_Osmotic-Cold_Stress/6357554

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