Infection of <i>Il17ra</i> KO mice with SRL1.

Abstract

<p>C57Bl/6 mice (9–11 per group) were infected with 10⁶ cfu/mouse SRL1 by intranasal inoculation and either observed for the course of the infection or culled after 24 hours. A. Kaplan Meier plot of survival for WT (solid line) or IL-17RA-/- (dashed line) (P < 0.01, log rank test). (B) Blood and (C) BALF bacterial counts 48 after infection. (D) BALF bacterial counts from mice which survived infection with SRL1 (pooled from multiple experiments). (E) Blood and (F) BALF neutrophils. (G) Weight of animals following infection; open circles IL-17RA KO and closed circles WT. Each point is the mean (n = 9–11); error bar is SEM. Difference between the groups is significant, (p < 0.001, two way ANOVA). (H) lung wet/dry mass ratio at 24 hours post infection. (I) Representative areas of inflamed lung from HE stained sections and neutrophil stained lung. (J) Lung inflammation measured by blinded assessment of HE stained sections. Significance levels as in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1007099#ppat.1007099.g002" target="_blank">Fig 2</a>. All data representative of two independent experiments.</p