In
this study, an interface coassembly strategy is employed to
rationally synthesize a yolk–shell CuO/silicalite-1@void@mSiO<sub>2</sub> composite consisting of silicalite-1 supported CuO nanoparticles
confined in the hollow space of mesoporous silica, and the obtained
composite materials were used as a novel nonenzymatic biosensor for
highly sensitive and selective detecting glucose with excellent anti-interference
ability. The synthesis of CuO/silicalite-1@mSiO<sub>2</sub> includes
four steps: coating silicalite-1 particles with resorcinol-formaldehyde
polymer (RF), immobilization of copper species, interface deposition
of a mesoporous silica layer, and final calcination in air to decompose
RF and form CuO nanoparticles. The unique hierarchical porous structure
with mesopores and micropores is beneficial to selectively enrich
glucose for fast oxidation into gluconic acid. Besides, the mesopores
in the silica shell can effectively inhibit the large interfering
substances or biomacromolecules diffusing into the void as well as
the loss of CuO nanoparticles. The hollow chamber inside serves as
a nanoreactor for glucose oxidation catalyzed by the active CuO nanoparticles,
which are spatially accessible for glucose molecules. The nonenzymatic
glucose biosensors based on CuO/silicalite-1@mSiO<sub>2</sub> materials
show excellent electrocatalytic sensing performance with a wide linear
range (5–500 μM), high sensitivity (5.5 μA·mM<sup>–1</sup>·cm<sup>–2</sup>), low detection limit
(0.17 μM), and high selectivity against interfering species.
Furthermore, the unique sensors even display a good capability in
the determination of glucose in real blood serum samples
