<p><i>Iris adriatica</i> Trinajstić ex Mitić (Iridaceae L.) is a strictly endemic taxon from Croatia. It is a rhizomatous dwarf plant from the <i>I. pumila</i> complex with a distribution area limited to the Croatian part of the Mediterranean area, mainly central Dalmatia. The genus <i>Iris</i> is known for its richness in isoflavonoids which also play a significant role in chemotaxonomy and biological activity. Hence, in the current study, different plant batches of <i>I. adriatica</i> collected in early spring of 2016 were analysed for their phytochemical profiles and qualitatively compared. UHPLC-PDA-ESI-MS analyses of methanolic rhizome extracts were performed. Altogether, 36 compounds, representing isoflavonoids (including 6,7-methylendioxy derivatives), benzophenones and xanthones were found as aglycones or in glycosidically bound form to be the main constituent groups of <i>I. adriatica</i> rhizomes. Qualitative results were identical between different batches of plant material from collection sites in central Dalmatia, they differed only in quantity. For some phenolic compounds of <i>I. adriatica</i>, chemotaxonomic relevance was detected.</p
