Effect of ι-carrageenan on transduction with PcPV1 and MfPV11.

Abstract

<p>(A) Different doses of ι-carrageenan were added to the cell culture medium immediately before transduction of HEK293TT cells with PcPV1 PsVs carrying a G.Luc reporter plasmid. Luciferase assay was performed 72h after transduction. Shown are mean values and standard deviation of three independent PcPV1 PsV preparations. Statistical analysis was performed by 2way ANOVA and Tukey’s multiple comparison test. (B) Additionally, HEK293TT cells were transduced with PcPV1 and MfPV11 PsVs carrying a pEGFP reporter plasmid with (+) and without (-) addition of 10μg/ml ι-carrageenan. GFP-positive cells were counted 72h after transduction and transducing units were calculated. Each data point represents one experiment with one PsV preparation. Dashed line indicates limit of detection. Statistical analysis was performed using t-test.</p