Validation of THBS1 knockdown in SCC25 and Cal27 cells. A. Relative mRNA expression of THBS1 in SCC25 and Cal27 after THBS1 knockdown (Scrambled as control), as determined by quantitative real-time PCR. Data are represented as the mean ± SD of three independent experiments, **p < 0.01. B. Relative protein expression of THBS1 in SCC25 and Cal27 after knockdown of THBS1 (Scrambled as control), as determined by Western blotting. Data are represented as the mean ± SD of three independent experiments, **p < 0.01. C. Expression level of THBS1 in CM of SCC25 and Cal27 after THBS1 knockdown (Scrambled as control), as determined by ELISA assays, **p < 0.01. D. Expression level of THBS1 in exosome supernatants of SCC25 and Cal27 cells after THBS1 knockdown (Scrambled as control), as determined by ELISA assays, **p < 0.01. (DOCX 199 kb
