Alteration of the sphingolipid metabolism under MAA-BSA treatment in WERI-Rb1 cells.

Abstract

<p><b>(A)</b> The sphingolipid pathway depicting the gene symbols of the enzymes involved (complete protein names in <b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0200739#pone.0200739.s002" target="_blank">S2 Table</a></b>). <b>(B-H)</b> Cells were treated with 80 μg/mL of BSA or MAA-BSA for 24 h, and gene expression or lipid levels were measured. <b>(B)</b> Genes from the <i>de novo</i> and salvage ceramide synthesis pathways. <b>(C)</b> Genes involved in the synthesis of SM from ceramides, and synthesis of glucosylceramides (GlcCer) and galactosylceramides (GalCer), both hexosylceramides (HexCer). <b>(D)</b> Genes involved in the generation of ceramide by sphingomyelin (SM) hydrolysis. <b>(E)</b> Ceramide (Cer) species levels. <b>(F)</b> SM species levels; only results from most abundant species are shown. <b>(G)</b> Ratio of total ceramide to total SM levels. <b>(H)</b> Ratio of total ceramide to total HexCer levels. Each value corresponds to the mean ± SEM of six-fold independently performed replicates evaluated in two batches. Statistics: Linear regression adjusted for batch of analysis; ***p<0.001, **p<0.01, *p<0.05.</p