<p>(<b>A</b>) Cells with the indicated genotypes carrying three YCp vectors (3YCp) or none (ctrl) were analyzed to determine cell size at budding. Individual data (<i>N</i> > 400), and median values are plotted. (<b>B</b>) Cells with the indicated genotypes carrying three YCp vectors were analyzed as in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2005388#pbio.2005388.g001" target="_blank">Fig 1B</a> to determine cell size at budding as a function of copy number. Individual budding volumes (small dots) were binned, and mean values (large circles, <i>N</i> = 50) and a regression line are plotted. Correlation analysis and pairwise comparisons were performed with nonparametric tests as described in Materials and methods. Underlying data can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2005388#pbio.2005388.s008" target="_blank">S1 Data</a>. CEN, centromere; YCp, yeast centromeric plasmid.</p