<p><b>(A)</b> Representative pictures of the <i>HvpPIN1a</i>:<i>HvPIN1a-mVENUS</i> expression in the outer cortex cell layer of the root meristem or in the epidermis of the SAM immediately after (0 h) or 2 h after mock (PBS) or 50 μM BFA treatment; gray arrow heads point to vesicles; scale bars 20 μm; three independent transgenic lines were examined; experiments were performed twice; n = 4–6; one transgenic line was used (in case of the root meristem); experiments were performed twice; n = 3–5; two transgenic lines were used (in case of shoot meristem). <b>(B)</b> Representative pictures of <i>HvpPIN1a</i>:<i>HvPIN1a-mVENUS</i> expression upon either mock (PBS, B)) or cytokinin (B’)) treatment as indicated in the captions; scale bar 200 μm. <b>(C)</b> Quantitative analysis of the <i>HvpPIN1a</i>:<i>HvPIN1a-mVENUS</i> expression upon cytokinin expression in B), measured by the mean gray value of the whole root meristem and the root cap; values are normalized to the PBS-control; five different independent transgenic lines were used; experiment was performed twice; n = 24 per treatment; significance was determined using the two-tailed Student’s t test, * = p<0.05.</p
