Absence of TRAIL⁺ ILC1s in NKp46-deficient mice.

Abstract

<p>(A) Percentages of TRAIL⁺ ILC1s were analyzed by flow cytometric analysis in the liver of <i>Ncr1</i>^gfp/gfp mice and their <i>Ncr1</i>^+/+ littermates. ILC1s were gated on Lin^─NK1.1⁺NKp46⁺(or GFP⁺ for <i>Ncr1</i>^gfp/gfp mice) TRAIL⁺CD49b^─ among lymphocytes. (B) Quantification of TRAIL⁺ ILC1s in the liver of <i>Ncr1</i>^gfp/gfp mice and their <i>Ncr1</i>^+/+ littermates for (A) (<i>n</i> = 5). (C) Quantification of TRAIL⁺ ILC1s in other organs (spleen, <i>n</i> = 5; BM, <i>n</i> = 5; SI, <i>n</i> = 4) of <i>Ncr1</i>^gfp/gfp mice and their <i>Ncr1</i>^+/+ littermates. **, <i>p</i> < 0.01; *, <i>p</i> < 0.05. The numerical data for panels B and C can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2004867#pbio.2004867.s007" target="_blank">S1 Data</a>. Lin^─, CD3^─CD19^─; BM, bone marrow; GFP, green fluorescent protein; ILC1, innate lymphoid cell 1; <i>Ncr1</i>, natural cytotoxicity receptor 1; NK, natural killer; SI, small intestine; TRAIL, tumor necrosis factor–related apoptosis-inducing ligand.</p