Pathogen challenge with <i>F</i>. <i>tularensis</i> LVS.

Abstract

<p>C57BL/6 mice (n = 7–10) were vaccinated 2 times, 28 days apart, with either TMV-DOST with adjuvant or with PBS + adjuvant. Mice were challenged with 10xLD₁₀₀ dose of <i>F</i>. <i>tularensis</i> LVS 21 days after the second vaccine and monitored for weight loss and survival. Mice were euthanized when weight loss exceeded 30%. (<b>A.</b>) Mice were vaccinated with TMV-DOST vaccine + CPG, or left untreated, and survival and body weights were recorded (<b>B.</b>) for 21 days. TMV-DOST was administered with di-GMP or left untreated and survival (<b>C.</b>) and body weights (<b>D.</b>) were recorded for 21 days. Statistical significance was determined via Kaplan-Meier Log-rank Test (GraphPad Prism).</p