IgG isotype profile analysis of TMV-DOST (D, DnaK;O, OmpA; S, SucB; T, Tul4) vaccinated mice.

Abstract

<p>Bleed 4 sera pooled from mice after the second immunization of 20 μg TMV-conjugated DOST antigen vaccine with (dIC, CpG, MF59 or diGMP) or without (neat) adjuvant was analyzed by ELISA against each antigen-using isotype specific IgG1 and IgG2b secondary antibodies to deduce type of immune response. Isotype profile is depicted as a ratio of the two, where direct addition gives total isotype content. <b>A</b>: Subcutaneous immunization profile. <b>B</b>: Intranasal immunization profile.</p