Recall IFNγ ELISpot and Granzyme B cellular immune response on D100.

Abstract

<p><b>A.</b> Splenocytes of twice-vaccinated mice were harvested at Day 100 after intranasal vaccination as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0194614#pone.0194614.g007" target="_blank">Fig 7</a>. (<b>A.</b>) Splenocytes from TMV-DOST vaccine groups were tested for Granzyme B secretion after antigen stimulation by the low responder (OmpA) and (<b>B.</b>) high responder (SucB) antigens given with no adjuvant (-), dIC, CpG or diGMP adjuvant. (<b>C</b>.) IFNγ responses after TMV-DOST + diGMP are compared to no stimulation, or PBS vaccinated mice (Norm) groups (2 mice per group). Values for both Granzyme B and IFNγ were adjusted positive spots per 10⁶ cells, and groups that show significant augmentation with adjuvants compared to no adjuvant are shown (** p<0.001 and *p<0.05). Means are presented ± standard deviation, and statistical analysis was done via non-parametric t-test.</p