Antigen-specific IgG titers post-vaccine 1 and 2 after TMV-DOST vaccination.

Abstract

<p>C57BL/6 mice were used in a subcutaneous (n = 4) and intranasal (n = 5) immunization with 20μg each TMV-conjugated tetravalent vaccine with or without (neat) adjuvant on day 1 (vaccine 1) and day 35 (vaccine 2). Sera was collected on day 28 (bleed 2) and day 63 (bleed 4) and tested for total IgG by ELISA on plates coated with 5μg of respective protein and quantified against a standard curve of antigen specific antibody. Post-vaccine 1 bleed 2 (b2) and post-vaccine 2 bleed 4 (b4) data is shown for Dnak (A), OmpA (B), SucB (C), and Tul4 (D). Means are presented ± standard deviation, where statistical significance is p<0.001 (*) compared to vaccination without adjuvant, using a nonparametric t-test.</p