MoMrt4 is important for vegetative growth, conidiation, and full virulence.

Abstract

<p>(A) Comparison of the wild type, the Δ<i>Momrt4</i> and the complement strains in vegetative growth on various medium. (B) The pathogenicity assay on rice leaves. Conidial suspensions of strains were sprayed onto two-week old rice seedlings (CO-39). Diseased leaves were photographed after 7 days of inoculation. (C) Quantification of lesion type. Lesions were photographed and measured at 7 days post-inoculation (dpi), counted within an area of 4 cm<sup>2</sup> and experiments were repeated three times with similar results. Asterisk indicates significant differences at p = 0.01. (D) Conidia development of the wild type, Δ<i>Momrt4</i> and the complement strains on SDC medium for 7 days were examined by light microscopy. (E) Statistical analysis of conidia production. Conidia produced by the wild-type, the mutant and complemented strains on SDC medium for 10 days were collected, counted and analyzed. ±SD is calculated from three repeated experiments and asterisks indicate statistically significant differences (Duncan’s new multiple range test, p < 0.01). (F) Ribosome proteins of indicated strains were extracted. Equal amounts of supernatant (S) and pellet (P) were separated by SDS-PAGE, and MoRpp0 and MoMrt4 were detected by Western blotting analysis using the anti-GFP and anti-FLAG antibodies, respectively.</p

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