Ionization efficiency improvement following aminoxyTMT labeling.

Abstract

<p>Injection of identical sample quantities before and after labeling resulted in increased signal intensity for numerous OS, as shown in the ESI mass spectra above for the OS of composition 3 Hex 6 HexNAc 1 Fuc (A–native OS; B–derivatized OS). Ions containing the aminoxyTMT label are annotated in blue; those without the label are shown in red. In-source fragments are annotated in green. OS are represented by their monosaccharide compositions, denoted as Hex_HexNAc_Fuc_NeuAc_NeuGc. 3’-SL, 3’-sialyllactose.</p