Methylation analysis of the promoter region in the <i>MLH1</i> gene.

Abstract

<p>(A) Schematic depiction of two regions (5’-region and 3’-region) of the <i>MLH1</i> promoter for methylation and results of a panel of representative fluorescent bisulfite PCR following restriction enzyme analysis. Methylated samples had the new fragment cleaved by the restriction enzyme. (B) The frequencies of <i>MLH1</i> promoter methylation according to <i>MLH1</i> expression status. The top panel shows the results of the <i>MLH1</i>-5’ region, the middle panel shows the <i>MLH1</i>-3’ region and the bottom panel shows partial (i.e. only <i>MLH1</i>-5’ methylation) and extensive methylation (i.e. both <i>MLH1</i>-5’ and -3’ methylation).</p