TO protein levels are increased in <i>slob^IP1</i> fly heads but still cycle.

Abstract

<p>TO protein levels in fly heads were measured by Western blot analysis and normalized to MAPK levels. <b><i>A</i></b><b>,</b> Representative Western blot showing an increase in TO expression in <i>slob^IP1</i> fly heads compared to <i>WT^P41</i> fly heads. TO levels are rescued in <i>slob^IP1,mai301>slob</i> fly heads. The graph is a summary of four independent experiments (mean ± SEM). * indicates p<0.05, One-way ANOVA with Bonferroni post-test. <b><i>B</i></b><b>,</b> Representative Western blot showing that TO levels cycle in <i>slob^IP1</i> fly heads. Zeitgeber time (ZT) is plotted on the X axis; the white and black bars represent “lights on” and “lights off”, respectively. The graph is a summary of three independent experiments (mean ± SEM).</p