DopEcR is required for the 30-minute courtship memory induced by 1-hour courtship conditioning.

Abstract

<p>(A) Thirty-minute courtship memory in wild-type flies (control) and flies heterozygous (<i>DopEcR^PB1</i>/+), homozygous (<i>DopEcR^PB1</i>/<i>DopEcR^PB1</i>), and hemizygous (<i>DopEcR^PB1</i>/Df(3L)ED4341) for <i>DopEcR</i>. <i>DopEcR^PB1</i> homozygotes and hemizygotes were defective for 30-minute courtship memory. (B) Time course of courtship memory in <i>DopEcR^PB1</i> homozygotes. Significant memory was observed immediately after conditioning, but not 15 or 30 minutes after conditioning. (C) A defect in 30-minute courtship memory in flies that ubiquitously express the <i>DopEcR</i> RNAi after eclosion, in response to RU486 stimulation of the <i>tub</i>-GS-Gal4 driver. The presence or absence of courtship memory was evaluated by applying the Mann–Whitney U-test to naïve and conditioned males. Statistical significance is shown above each bar as NS, no significant difference, **, <i>P</i><0.01 or ***, <i>P</i><0.001. Sample numbers for naïve and conditioned flies are shown under each graph. PIs were analyzed using Krustal-Wallis One-Way ANOVA, followed by Dunn's pairwise test for multiple comparisons. #, <i>P</i><0.05; ##, <i>P</i><0.01. Error bars (s.e.m.).</p