The ADAR2-mediated editing at specific nucleotide residues in the RNA transcripts related with pri-miR-214 and pri-miR-122 also occurred in the clinical HCC specimens.

Abstract

<p>The representative sequencing results for miR-214 (<b>A</b>) and miR-122 (<b>B</b>), with the RT–PCR products from four pairs of HCC tissues as indicated. The nucleotide residues with changes in the tumorous (T) tissues, in comparison with those in the corresponding nontumorous (NT) tissues, are marked with red arrows. The positions relative to that of the mature miRNAs are indicated above the arrows. (<b>C</b>) The relative expression levels of ADAR2 mRNA in these HCC tissues were determined by qPCR analysis, with the quantitative results shown as relative to the level of the NT part of patient no. 8. (<b>D</b>) The protein expression of ADAR2 and ADAR1 in these four pairs of HCC tissues was examined by Western blotting.</p