Heatmaps of the differences in the Pearsonâs r correlation coefficient between survivors and nonsurvivors. Lipids are arranged based on hierarchical clustering using a complete linkage algorithm. The dendrogram shows how lipids were arranged relative each other. The group of lipids that has a greater correlation in survivors (red font) contains most of the lipids that had a greater concentration in those who died and contains many lipids that originate from the cell membrane. The cluster with the greatest amount of correlation among non-survivors (yellow highlighting) contains primarily glycerolipids consisting mostly of polyunsaturated fatty acids. These observations suggest that these groups of lipids play an important role for the outcome of patients with ARDS. (PDF 119Â kb

Charles Burant (5085962)

Elizabeth Jewell (5085959)

Kathleen Stringer (5085965)

Michael Maile (4605799)

Milo Engoren (4250092)

Tanu Soni (5085971)

Thekkelnaycke Rajendiran (5085974)

Theodore Standiford (5085968)

Abstract
              
                Background
                It is unknown if the plasma lipidome is a useful tool for improving our understanding of the acute respiratory distress syndrome (ARDS). Therefore, we measured the plasma lipidome of individuals with ARDS at two time-points to determine if changes in the plasma lipidome distinguished survivors from non-survivors. We hypothesized that both the absolute concentration and change in concentration over time of plasma lipids are associated with 28-day mortality in this population.
              
              
                Methods
                Samples for this longitudinal observational cohort study were collected at multiple tertiary-care academic medical centers as part of a previous multicenter clinical trial. A mass spectrometry shot-gun lipidomic assay was used to quantify the lipidome in plasma samples from 30 individuals. Samples from two different days were analyzed for each subject. After removing lipids with a coefficient of variation > 30%, differences between cohorts were identified using repeated measures analysis of variance. The false discovery rate was used to adjust for multiple comparisons. Relationships between significant compounds were explored using hierarchical clustering of the Pearson correlation coefficients and the magnitude of these relationships was described using receiver operating characteristic curves.
              
              
                Results
                The mass spectrometry assay reliably measured 359 lipids. After adjusting for multiple comparisons, 90 compounds differed between survivors and non-survivors. Survivors had higher levels for each of these lipids except for five membrane lipids. Glycerolipids, particularly those containing polyunsaturated fatty acid side-chains, represented many of the lipids with higher concentrations in survivors. The change in lipid concentration over time did not differ between survivors and non-survivors.
              
              
                Conclusions
                The concentration of multiple plasma lipids is associated with mortality in this group of critically ill patients with ARDS. Absolute lipid levels provided more information than the change in concentration over time. These findings support future research aimed at integrating lipidomics into critical care medicine.https://deepblue.lib.umich.edu/bitstream/2027.42/143134/1/12931_2018_Article_758.pd

Maile, Michael D

Standiford, Theodore J

Engoren, Milo C

Stringer, Kathleen A

Jewell, Elizabeth S

Rajendiran, Thekkelnaycke M

Soni, Tanu

Burant, Charles F

Deep Blue Documents

Associations of the plasma lipidome with mortality in the acute respiratory distress syndrome: a longitudinal cohort study

Abstract Background It is unknown if the plasma lipidome is a useful tool for improving our understanding of the acute respiratory distress syndrome (ARDS). Therefore, we measured the plasma lipidome of individuals with ARDS at two time-points to determine if changes in the plasma lipidome distinguished survivors from non-survivors. We hypothesized that both the absolute concentration and change in concentration over time of plasma lipids are associated with 28-day mortality in this population. Methods Samples for this longitudinal observational cohort study were collected at multiple tertiary-care academic medical centers as part of a previous multicenter clinical trial. A mass spectrometry shot-gun lipidomic assay was used to quantify the lipidome in plasma samples from 30 individuals. Samples from two different days were analyzed for each subject. After removing lipids with a coefficient of variation > 30%, differences between cohorts were identified using repeated measures analysis of variance. The false discovery rate was used to adjust for multiple comparisons. Relationships between significant compounds were explored using hierarchical clustering of the Pearson correlation coefficients and the magnitude of these relationships was described using receiver operating characteristic curves. Results The mass spectrometry assay reliably measured 359 lipids. After adjusting for multiple comparisons, 90 compounds differed between survivors and non-survivors. Survivors had higher levels for each of these lipids except for five membrane lipids. Glycerolipids, particularly those containing polyunsaturated fatty acid side-chains, represented many of the lipids with higher concentrations in survivors. The change in lipid concentration over time did not differ between survivors and non-survivors. Conclusions The concentration of multiple plasma lipids is associated with mortality in this group of critically ill patients with ARDS. Absolute lipid levels provided more information than the change in concentration over time. These findings support future research aimed at integrating lipidomics into critical care medicine

Michael D. Maile

Theodore J. Standiford

Milo C. Engoren

Kathleen A. Stringer

Elizabeth S. Jewell

Thekkelnaycke M. Rajendiran

Tanu Soni

Charles F. Burant

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Table S2. Area under the receiver operating characteristic curve for lipids that differ between survivors and non-survivors. (DOCX 35Â kb

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Additional file 3: of Associations of the plasma lipidome with mortality in the acute respiratory distress syndrome: a longitudinal cohort study

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