<p>(A) LAMP targeting the <i>polA</i> gene. All <i>TP</i> strains become positive. (B) LAMP assay targeting the <i>tprL</i> locus, which results in amplification of <i>TPA</i> and <i>TEN</i> strains. (C) Only <i>TPE</i> and <i>TEN</i> strains generate positive results in the LAMP assay targeting the TP_0619 locus. Note that the strain material in the three assays had different <i>TP</i> copy numbers as indicated in <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0006396#pntd.0006396.s004" target="_blank">S1 Table</a>. Data points are presented as mean±SEM values. Red cross = Negative control; <i>TPA</i> strains (blue) up-pointing triangle = Mexico A, down-pointing triangle = SS14, circle = Nichols, square = Seattle 81–4; <i>TPE</i> strains (green) square = Gauthier, square turned = Sei Geringing K403, hexagon = Kampung Dalan K363, circle = Samoa D, circle with cross = CDC-1, square with cross CDC-2; <i>TPE</i> simian strains (purple) cross = Fribourg-Blanc, star = RNP; <i>TEN</i> strains (black) square = Bosnia A, circle with cross = Iraq B.</p
