Ackermann, GL

Amir, A

Astudillo-Garcia, C

Bell, JJ

Bjork, JR

Cerrano, C

Costa, R

Dailianis, T

Easson, C

Erwin, PM

Feng, G

Gonzalez, A

Hentschel, U

Hill, RT

Ilan, M

Knight, R

Kotoulas, G

Li, Z

Liu, F

Lopez-Legentil, S

Marra, MV

McCormack, GP

Moitinho-Silva, L

Montoya, JM

Nielsen, S

Olson, JB

Pineda, M-C

Polymenakou, P

Ravasi, T

Reveillaud, J

Schupp, PJ

Simister, RL

Sipkema, D

Steindler, L

Steinert, G

Taylor, MW

Thacker, RW

Thomas, T

Vicente, J

Webster, NS

Marine sponges (phylum Porifera) are a diverse, phylogenetically deep-branching clade known for forming intimate partnerships with complex communities of microorganisms. To date, 16S rRNA gene sequencing studies have largely utilised different extraction and amplification methodologies to target the microbial communities of a limited number of sponge species, severely limiting comparative analyses of sponge microbial diversity and structure. Here, we provide an extensive and standardised dataset that will facilitate sponge microbiome comparisons across large spatial, temporal, and environmental scales. Samples from marine sponges (n = 3569 specimens), seawater (n = 370), marine sediments (n = 65) and other environments (n = 29) were collected from different locations across the globe. This dataset incorporates at least 268 different sponge species, including several yet unidentified taxa. The V4 region of the 16S rRNA gene was amplified and sequenced from extracted DNA using standardised procedures. Raw sequences (total of 1.1 billion sequences) were processed and clustered with (i) a standard protocol using QIIME closed-reference picking resulting in 39 543 operational taxonomic units (OTU) at 97% sequence identity, (ii) a de novo clustering using Mothur resulting in 518 246 OTUs, and (iii) a new high-resolution Deblur protocol resulting in 83 908 unique bacterial sequences. Abundance tables, representative sequences, taxonomic classifications, and metadata are provided. This dataset represents a comprehensive resource of sponge-associated microbial communities based on 16S rRNA gene sequences that can be used to address overarching hypotheses regarding host-associated prokaryotes, including host specificity, convergent evolution, environmental drivers of microbiome structure, and the sponge-associated rare biosphere

Moitinho-Silva, Lucas

Nielsen, Shaun 

Amir, Amnon

Gonzalez, Antonio

Ackermann, Gail L

Cerrano, Carlo

Astudillo-Garcia, Carmen

Easson, Cole

Sipkema, Detmer

Liu, Fang

Steinert, Georg

Kotoulas, Giorgos

McCormack, Grace P

Feng, Guofang

Bell, James J

Vicente, Jan

Bjork, Johannes R

Montoya, Jose M

Olson, Julie B

Reveillaud, Julie

Steindler, Laura

Pineda, Mari-Carmen

Marra, Maria V

Ilan, Micha

Taylor, Michael W

Polymenakou, Paraskevi

Erwin, Patrick M

Schupp, Peter J

Simister, Rachel L

Knight, Rob

Thacker, Robert W

Costa, Rodrigo

Hill, Russell T

Lopez-Legentil, Susanna

Dailianis, Thanos

Ravasi, Timothy

Hentschel, Ute

Li, Zhiyong

Webster, Nicole S

Thomas, Torsten 

UNSWorks

The sponge microbiome project

The sponge microbiome project

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