Normal blood cell counts in peripheral blood and normal CXCL12 levels in serum indicate proper CXCL12/CXCR4 signaling in HuCXCR4KI mice.

Abstract

<p>Each symbol represents the result from an individual mouse. <b>A</b>, Flow cytometric analysis of total leukocyte (WBCs) and leukocyte subset counts in peripheral blood of HuCXCR4KI and WT control littermate mice. Cell counts were estimated with absolute counting beads, as described in the methods section. WBCs were defined as CD45⁺ blood cells. WBC subsets were defined based on cell surface marker expression, as follows: monocytes (CD45⁺CD11b⁺CD115⁺Ly6G^-), neutrophils (CD45⁺CD11b⁺CD115^-Ly6G⁺), T cells (CD45⁺CD11b^-Thy1.2⁺), B cells (CD45⁺CD11b^-CD19⁺), NK cells (CD45⁺CD11b^-CD49b⁺). <b>B</b>, Detection by ELISA of CXCL12 levels in the serum of HuCXCR4KI and WT control littermate mice; n.s. = non-significant (unpaired Student’s <i>t</i>-test).</p