Expression of AmCDase in the yeast double knockout strain Δypc1Δydc1.

Abstract

<p>a) Determination of whole-cell lysates (20 μg protein per lane) by Western blot with anti-His antibody at different time points. b) D-erythro-C12-NBD-ceramide content in different groups after the reaction. Values are the means ± SD of three replicates from each independent experiment.</p