Figure S1 from Structural and functional studies of Spr1654: an essential aminotransferase in teichoic acid biosynthesis in <i>Streptococcus pneumoniae</i>
Absorption spectrum of Spr1654 and Spr1654-PLP. (a) Analysis of recombinant Spr1654-his (MW 47.7 kDa) by SDS-gel electrophoresis. The protein was separated by SDS-12% PAGE, followed by staining with Coomassie blue. Lane 1, Spr1654-his; M, molecular mass marker. (b) The absorption spectra of Spr1654 with (red line) and without PLP (blue line) were collected between 300 and 500 nm. The appearance of a 420nm peak indicates PLP binding to Spr1654