Additional file 5: Figure S3. Differential expression of genes found in Clostridia sporulation cascades. pro-σE processing protease is a stage III sporulation factor. BofA is an inhibitor of the stage IV pro-σK processing protease SpoIVFB. Table S2. Percentage of spherical morphologies 144 and 216 h after inoculation. Figure S4. Substrates and products (A) and the pH (B) after 144 and 216 h of C. thermocellum-mutant fermentations on MOPS-free carbon-replete medium starting with an initial pH of 6.75. Significant differences at α = 0.001 for comparisons with DSM1313 (∆hpt) are indicated with a “*” and comparisons with DSM1313 (∆hpt) and LL1210 are indicated with “**”. Averages were calculated with six biological replicates. Error bars indicate standard deviation