The pre-OR region of PrP^ScΔOR is PK-resistant.

Abstract

<p>(A) The brain homogenates of terminally ill wild-type and tg(PrPΔOR)/<i>Prnp^0/0</i> mice were treated with PNGase F after digestion with PK, and subjected to immunoblotting with M-20 anti-PrP antibodies. The deglycosylated PK-resistant band of PrP^ScΔOR was higher in molecular size than that of full-length PrP^Sc. Arrows indicates PK-resistant deglycosylated PrPs. (B) The brain homogenates from terminally ill wild-type and tg(PrPΔOR)/<i>Prnp^0/0</i> mice were digested with PK, and subjected to immunoblotting with N-terminus-specific IBL-N anti-PrP antibody. The IBL-N antibodies recognized the PK-resistant PrPs from PrP^ScΔOR but not from full-length PrP^Sc.</p