Alignment of the human GAPDH mRNA with seven PGs that are the best homologous to the wild type GAPDH mRNA shows that the first 26 nt of the mRNA is the only unique region while the 685–705th nt region of the GAPDH has the most mismatches (shown as underlined lowercase letters in PGs).

Abstract

<p>We select the 6–25th and the 685–705th regions as the forward and reverse primers (underlined), respectively.</p