Overview of a linear mRNA amplification based on the procedure described by Wang

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Options available for profiling small samples: a review of sample amplification technology when combined with microarray profiling"</p><p>Nucleic Acids Research 2006;34(3):996-1014.</p><p>Published online 9 Feb 2006</p><p>PMCID:PMC1363777.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> (). Following oligo dT priming, the method exploits the template switching effect of the reverse transcriptase enzyme. The RT enzyme incorporates non-template dCTPs at the 3′ end of the transcript, then switches templates and continues replication to the end of the primer. The result is full length cDNA. For the second strand, a primer with bases complementary to the dCTP stretch is applied. Antisense RNA is transcribed by the T7 RNA polymerase