Effect of the BRCA1 452–1079 amino acids fragment on WRN helicase and exonuclease activities

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Collaboration of Werner syndrome protein and BRCA1 in cellular responses to DNA interstrand cross-links"</p><p>Nucleic Acids Research 2006;34(9):2751-2760.</p><p>Published online 19 May 2006</p><p>PMCID:PMC1464112.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> () WRN helicase activity was measured as described in using a 22 bp forked duplex substrate. () WRN exonuclease activity was measured on a 34 bp forked duplex substrate. Purified WRN was pre-incubated with BRCA1 452–1079 amino acids at the indicated concentrations in 10 µl assay buffer. The mean percent strand displacement or digestion ( = 2) on the substrate is shown