Gene expression profiling, for genes involved in regulating apoptosis, in A549, MCF-7 and HepG2 cells incubated with or without etoposide under normoxic or hypoxic conditions

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Differential effects of hypoxia on etoposide-induced apoptosis according to the cancer cell lines"</p><p>http://www.molecular-cancer.com/content/6/1/61</p><p>Molecular Cancer 2007;6():61-61.</p><p>Published online 26 Sep 2007</p><p>PMCID:PMC2099441.</p><p></p> Please refer to supplementary data [Additional file ] for results obtained for the 62 genes for which there was a significant variation in expression for at least one of the conditions. Cells were incubated under normoxic (N) or hypoxic (H) conditions with or without etoposide (e, 50 μM) for 16 hours before RNA extraction, reverse-transcription and cDNA hybridization, as described in Materials and Methods. Each value is the average of three ratio values calculated from three independent experiments ± 1 S.D. Mean ratios indicate a fold-increase or decrease in gene expression. Qualitative values are given with + or - signs (according to the inserted table). The red vertical bars correspond to undetected cDNA. Duplicates or unique value are noted with a red 2 or 1 behind the corresponding column

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