<p><b>Copyright information:</b></p><p>Taken from "Merr. suppresses the induction and progression of autoimmune arthritis by modulating immune response to heat-shock protein 65"</p><p>http://arthritis-research.com/content/9/4/R70</p><p>Arthritis Research & Therapy 2007;9(4):R70-R70.</p><p>Published online 23 Jul 2007</p><p>PMCID:PMC2206370.</p><p></p> Two groups of LEW rats (= 6 to 9) were fed either Celastrus (3 g/kg) or water as described in the legend to Figure 1. A sub-group of these LEW rats was euthanized on day 12 after H37Ra immunization, and the draining LNCs of these rats were cultured in a 96-well plate in the presence of the indicated recall antigens (HEL, hen eggwhite lysozyme; B177, synthetic peptide 177–191 of Bhsp65). The supernatant was collected after 72 h of cell culture and tested in ELISA for IFN-γ and IL-10. The results are expressed as pg/ml (mean + standard error of the mean). The difference in the level of IL-10 but not of IFN-γ in response to Bhsp65 in Celastrus-fed versus water-fed rats is statistically significant (*< 0.05). The mean IFN-γ/IL-10 ratio in response to Bhsp65 of Celastrus-treated (8.79) rats was significantly reduced compared to that of the water-fed (20.76) rats
